There are various AAV serotypes, and in cardiac studies, AAV1, AAV2, AAV6, AAV8, and AAV9 have good infection efficiency in the heart, with AAV8 and AAV9 being better than the other serotypes, and AAV9 is currently recognized as a highly efficient and specific targeting of the cardiac expression of the viral vector serotype. Although AAV8 was also effective in infection, it did not spread as well as AAV9. The serotypes that target the myocardium in clinical practice are generally 1, 6, and 9, of which AAV1 and AAV6 are both effective in targeting cardiac and skeletal muscle cells, whereas AAV9 is more inclined to infect the myocardium.