Creative Biogene provides a comprehensive service, focusing on reporter gene knockin, tag knockin, and fragment knockin. Our service encompasses everything from the design of gRNA (guide RNA), vector construction, cell transfection and selection, to single clone sequencing and cell expansion. Our skilled scientists are proficient in generating knockin cell lines using the CRISPR system, ensuring high-quality results and exceptional service.

Importance of Knockin Cell Lines
Knockin cell lines, which involve inserting a specific fragment at a designated locus within the genome, serve as crucial tools for research. By inserting a reporter gene or tag at a specific locus, researchers can study the endogenous expression levels of target genes. The CRISPR/Cas9 system simplifies the creation of custom knockin cell lines by utilizing the Cas9 nuclease, which targets specific genomic loci through sgRNA, inducing double-strand breaks. These breaks can be repaired via the HDR (homology-directed repair) pathway when a template is present, allowing for the insertion of promoters, tags, or complete genes into the genome.

Our Services
Types of Knockin Cell Lines
Conventional Knock-in Cell Line:

Here, a reporter gene is inserted at a specific site and driven by an endogenous promoter, which reflects the expression of the targeted gene.

Conditional Knock-in Cell Line:

This involves using an inducible knockin cassette, commonly with a Cre/loxP system, allowing the inserted gene to be activated in the presence of Cre recombinase.
Workflow
The generation of knockin cell lines follows a systematic workflow, ensuring meticulous execution of all essential steps.

Why Choose Us?
At Creative Biogene, we specialize in generating knock-in cell lines across various cell types, including tumor and stem cell lines. Our advanced CRISPR platform and dedicated team of scientists are committed to expediting your research projects by providing comprehensive support throughout the process.