Primary cells only undergo a pre-determined and finite number of cell divisions in culture. After limited population doublings (the number of which varies by species, cell type, and culture conditions), primary cells enter a state, so-called replicative senescence, where they can no longer divide.

Immortalizing the cell line will eliminate the frequent need to re-establish fresh cultures from specimens. Immortalized cell lines are capable of extended proliferation and should possess similar or identical genotype and phenotype to the parental tissue.

To surpass senescence, Creative Bioarray have developed several HT® Cell Immortalization Kit for immortalizing mammalian cells in culture. Also check out our existing collection of Immortalized Cells or our Cell Immortalization Service.