In the field of RNA biology, circular RNAs (circRNAs) have emerged as significant players in various cellular processes, influencing gene expression and regulating the function of other RNA molecules, particularly microRNAs (miRNAs). At Creative Biogene, we offer advanced circRNA Luciferase Reporter Assay services tailored for rigorous circRNA research, ensuring high-quality results for our clients.

Understanding circRNA and Its Importance
CircRNAs are unique entities characterized by their covalently closed structure, formed through the back-splicing of precursor mRNAs. Recent innovations in RNA sequencing and bioinformatics have led to the identification of thousands of circRNAs across different species. These molecules have been found to interact with proteins and other RNA species, acting as transcriptional regulators or even serving as miRNA sponges. This interaction landscape makes circRNAs crucial for understanding cellular mechanisms and potential therapeutic targets.

The Role of Luciferase Reporter Assays
To elucidate the functional dynamics of circRNAs, particularly in their interaction with miRNAs, the circRNA Luciferase Reporter Assay serves as a fundamental tool. Here’s how it works:

Constructing the Reporter Vector: A luciferase vector (e.g., psiCHECK2) is engineered by inserting the circRNA sequence into the 3’ untranslated region (UTR) of a promoter-driven luciferase gene.

Co-transfection: Cells are co-transfected with both the luciferase reporter construct and miRNA mimics.

Luciferase Activity Measurement: By comparing the luciferase activity in experimental groups with negative controls, researchers can determine the interaction efficiency between circRNAs and miRNAs. A decrease in luciferase expression suggests effective binding and regulation by the miRNA.

Validation of Binding Sites: By using mutated circRNA sequences, researchers can verify specific binding sites that interact with miRNAs, further unraveling the circRNA regulatory network.

Advantages of Our circRNA Luciferase Reporter Assay
Using our advanced tools and methodologies, we aim to provide insights that facilitate both basic research and drug discovery. The key advantages of our service include:

Instantaneous Measurements: Gain timely insights into circRNA-miRNA interactions.
Exceptional Sensitivity: Detect even subtle changes in luciferase activity, enhancing the reliability of your findings.
Dynamic Range: Suitable for a wide array of cell lines to ensure comprehensive experimental validation.
Streamlined Process: We offer a one-stop solution with an efficient workflow and quick turnaround time.
Get Started with Your Research
As a leading biotechnology company specializing in RNA biology, Creative Biogene is equipped with skilled professionals who are dedicated to supporting your circRNA research needs. With our circRNA Luciferase Reporter Assay service, simply provide the sequence information of the circRNA and/or the predicted miRNA, and we will deliver reliable experimental results tailored to your research objectives.