In this situation it is really important to follow special steps and regulations. I recommend to read special literature about it.

To avoid any loss of data, you must be very accurate with it. Every step in the analysis is crucial, and even small errors can cast doubt on the results. I’ve personally encountered this issue in my practice: even if the sample seems small, accurate data is needed for analysis, and if something goes wrong, all the work can be wasted. Modern RNA quantification methods allow working with minimal volumes while maintaining confidence in the accuracy of the results. These technologies speed up the process while maintaining a high degree of data reliability. Similar rna quantification solutions can be found on berthold.com and significantly increase the speed and accuracy of diagnostics. In practice, this means not only accelerating laboratory work but also potential savings on reagents, which is especially important in the long term.